Esta web utiliza cookies. Si continúas navegando consideramos que aceptas su uso.
Más información
Aceptar
I Jornadas Científicas del IMIB-Arrixaca
Acceso Personal
Contacto
Aviso Legal
Inicio
Bienvenida
Comités
Ponencias
Comunicaciones
Programa
Fechas clave
Inscripciones
Selección comunicaciones
Enviar comunicación
Patrocinadores
Sede
Imprimir
LEYDIG CELL LOSS DURING AGING AND AFTER EXPOSURE TO A SHORT PHOTOPERIOD IN THE TESTES OF SYRIAN HAMSTER
Autores:
ESTER BELTRÁN FRUTOS
, JESÚS MARTÍNEZ HERNÁNDEZ,
VICENTE SECO ROVIRA
,
MARIA CONCEPCION FERRER CAZORLA
,
LUIS MIGUEL PASTOR GARCÍA
,
Grupos de investigación:
[GI/IMIB/C012/2011] Integración morfofuncional de células y tejidos
[GI/IMIB/C007/2011] Biología de la Reproducción
Comunicación:
Antecedentes:
The population of Leydig cells is responsible in part of endocrine function in the testis by synthesizing and secreting the major circulating androgen: the testosterone, which has multiple functions in the body. The morphology and the number of this cell vary depending on the species, age and reproductive seasonality. While several authors refer to the decrease in testosterone with aging in men or after exposure to a short photoperiod in hamsters, the cellular mechanisms involved in this lower production are not known. Most authors agree that decreasing the hormone testosterone is associated with a decrease in the number of Leydig cells with age in humans (Kaler and Neaves 1978), pigs (Tripepi et al., 2000), and in hamsters subject to photoperiod short (Hardy et al., 1987; Sinha Hikim et al., 1988). The principal aim of our work is to investigate the cellular mechanism which is involved in the decrease of number Leydig cells associated with aging and testicular regression due to short photoperiod in the Syrian hamster.
Métodos:
A total of 24 hamsters were used. All animals, except the controls, were subjected to an 8:16h (light/dark) photoperiod. After 4, 6, 8, 10 and 12 weeks the animals were sacrificed. Also we used a total of 32 animals in long photoperiod 16:8h (light/dark), eight 6 months old, eight 12 months old and seven 24 months old for aging study. The testes were fixed in methacarn and glutaraldehyde and osmium tetroxide for light microscopy and transmission electron microscopy respectively. These cells were studied histochemically with regard to their proliferation (Proliferation Cell Nuclear Antigen- PCNA) and apoptosis (terminal deoxynucleotidyl transferase (TdT)-mediated dUTP in situ nick end labeling-TUNEL). The index of Leydig cells positive for PCNA and TUNEL+ was determined. The total number of cells positive for PCNA and TUNEL+ and total Leydig cells was quantified. Appropriate statistical analyses were performed.
Resultados:
No important changes in the proliferation and apoptosis of Leydig cells during regression and aging were observed The Leydig cells suffered few ultrastructural changes during aging. and necrotic Leydig cells were observed. In contrast, an ultrastructural involution of Leydig cells together with necrosis cells was observed in the regressed group. The index and number of necrotic cells was increased during the initial regression. The results obtained during aging and during regression after exposure to a short photoperiod suggest that the loss of Leydig cells would be produced by a controlled necrosis phenomenon that could be related to necroptosis. Control and regulation of this phenomenon may be a way to keep the endocrine function of the testis in advanced ages or mitigate the loss of these cells in certain testicular pathologies. Funded by GERM 19892/15 from Fundación Seneca CARM.
Conclusiones:
Instituto de Investigación Sanitaria Acreditado
Inicio
Grupo de Investigación
Miembros
Proyectos
Colaboraciones
Servicios
Recursos formativos
Producción Científica
Publicaciones
Tesis
Novedades
Noticias
Eventos
Convocatorias
Agenda