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PROLIFERATIVE AND APOPTOTIC CHANGES IN THE SEMINIFEROUS EPITHELIUM OF SYRIAN HAMSTER DURING THE TESTICULAR RECRUDESCENCE
Autores:
VICENTE SECO ROVIRA
, JESUS MARTÍNEZ HERNANDEZ,
ESTER BELTRÁN FRUTOS
,
MARIA CONCEPCION FERRER CAZORLA
,
LUIS MIGUEL PASTOR GARCÍA
,
Grupos de investigación:
[GI/IMIB/C012/2011] Integración morfofuncional de células y tejidos
[GI/IMIB/C007/2011] Biología de la Reproducción
Comunicación:
Antecedentes:
After exposure to short photoperiod in the Syrian hamster a testicular regression with the involution of the seminiferous epithelium occurs. The restoration of complete spermatogenesis occurs spontaneously during recrudescence. The objective of this study was to ascertain how the phenomena of apoptosis and proliferation affect the seminiferous epithelium during recrudescence.
Métodos:
27 male hamsters were used (21 treated and 6 controls (CT)). The treated animals were subjected to an 8:16 light-dark photoperiod while the control animals were subjected to a 14:10 light-dark photoperiod. Seven treated animals plus two from the control group were sacrificed at 16, 19 and 21 weeks. Testes were fixed in methacarn and embedded in paraffin. Three recrudescence groups were established: Initial (IR), Advanced (AR) and Total (TR). The proliferative activity was determined by the PCNA immunohistochemistry. The apoptotic activity was determined by TUNEL histochemistry. The proliferation index of spermatogonia (PI), the apoptotic indexes (AI) of spermatogonia (AI-SG), spermatocyte (AI-SC), round spermatids (AI-SP), AI-SG+SC and AI-SG+SC+SD and the ratio between cells in apoptosis (A) and spermatogonia in proliferation (SG-P) were calculated. Also the total number of each germ cell ((TN-SG), (TN-SC) and (TN-SD)) and the total number (TNA) of each apoptotic germ cells ((TNA-SG), (TNA-SC) and (TNA-SD)) were calculated. To study the apoptosis and proliferation of Sertoli cells, TUNEL, PCNA and vimentin immunohistochemistry for light, fluorescence and confocal microscopy were performed. The apoptosis and proliferative indexes of Sertoli cells (AI-S and PI-S) and the total, apoptotic and proliferative numbers of Sertoli cells were calculated.
Resultados:
With respect to the germ cells, the AI of each germ cell showed that in IR was higher than in the other groups. The TN of each germ cell was lower in IR than in the other groups. Only the TNA-SC was higher in IR and AR than in the other groups. Regarding to the TN of germ cells (SG+SC+SD) it was lower in IR compared to other groups, while the TNA (SG+SC+SD) was higher in IR and AR than in TR. The AI-SG+SC and AI-SG+SC+SD were highest in IR. The PI was higher in all groups of recrudescence compared with the CT not finding significant differences between them. Only the SG-A/SG-P ratio did not show changes during recrudescence. The other ratios were higher in IR with respect to TR and CT. TUNEL+ and vimentin + cells in all groups, which corresponded to Sertoli cells in apoptosis were observed. No significant differences between the groups studied as regards the AIS were found. PI-S was increased in IR group and total Sertoli cells signiticatively decreased in this group.
Conclusiones:
In conclusion during recrudescence: a) The SG population has similar apoptotic activity to CT while the proliferative activity is greater; b) in the IR group, a substantial apoptotic activity remains in SC and SD while it decreases in AR allowing to recover the complete spermatogenesis in the seminiferous epithelium; d) the balance between proliferative and apoptotic activities that exists during the breeding period is reestablished halfway through the recrudescence period, e) the apoptosis and proliferation of Sertoli cells was observed under normal physiological conditions ; f) the AI of Sertoli cell remained stable throughout the recrudescence process and g) Sertoli cell proliferation restores their total number during the process of recrudescence.
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