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NEUROPROTECTIVE EFFECTS OF SEVERAL NEUROTROPHIC FACTORS IN A FOCAL LIGHT-EMISSION-DIODE INDUCED PHOTOTOXICITY MODEL
Autores:
FRANCISCO JAVIER VALIENTE SORIANO
,
DIEGO GARCIA AYUSO
,
ARTURO ORTIN MARTINEZ
,
MANUEL JIMENEZ LOPEZ
, JOSÉ MANUEL BERNAL GARRO, PATRICIA BECERRA,
MARÍA PAZ VILLEGAS PÉREZ
,
MANUEL VIDAL SANZ
,
Grupos de investigación:
[GI/IMIB/C004/2011] Oftalmología Experimental
Comunicación:
Antecedentes:
To investigate the neuroprotective effects of the neurotrophic factors, Brain-derived-neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF) or different doses of pigment epithelium-derived factor (PEDF), in a LED induced phototoxicity (LIP) photoreceptor degeneration model.
Métodos:
Adult female Swiss mice (25-35 g) were dark adapted overnight, anesthetized and their left pupil dilated with topical tropicamide (1%). A 10V blue (400nm) LED was placed at 1mm from the cornea and the eye exposed for 20sec (200lux). LIP damage was assessed in retinas inmunodetected 7 days after LIP against S-opsin, which identifies approximately 96% of the entire cone population. The effects of a single intravitreal injection of BDNF (2.5µg), CNTF (0.2µg), PEDF (0.1, 0.3 or 0.5µg) or bFGF (0.5µg) after LIP in the S-cone population were examined on flat-mounted retinas at 7 days. Total numbers of S-cones were counted automatically within a circular area (with a radius of 0.4 mm) centered on the lesion in the left retinas and in a corresponding region of their fellow right retinas, which served as controls.
Resultados:
Wholemounts showed a circular region located 0.8 mm from the optic disk in the superotemporal quadrant with diminished numbers of S-cones. In this area the number of S-cones were 2,074±449 (n=8) for the LED exposed retinas treated with PBS while there were 5,716±550 (n=8) for the contralateral fellow retinas, that is a loss of approximately 36.3% of the original population. The following intravitreal treatments showed a significant neuroprotective effects on the S-cone population, when compared with their corresponding vehicle groups, with greater survival: BDNF (56.2%; n=8), bFGF (50.3%; n=8), PEDF 0.5µg (50.7%, n=8). However CNTF (42.6%; n=7), PEDF 0.3 µg (37.7%; n=7) and PEDF 0.1 µg (36.7%; n=7) did not show protection.
Conclusiones:
The focal loss of cones caused by LIP in the retina is a reliable model that can be rescued significantly with intravitreal BDNF, PEDF or bFGF in albino mice.
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