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SILK-FIBROIN AND GRAPHENE PROMOTE PERIODONTAL LIGAMENT STEM CELLS DIFFERENTIATION INTO OSTEO/CEMENTOBLAST-LIKE CELLS
Autores:
FRANCISCO JAVIER RODRÍGUEZ LOZANO
, MAR VERA SÁNCHEZ, SALVADOR AZNAR CERVANTES,
EVA JOVER GARCÍA
,
DAVID GARCÍA BERNAL
, RICARDO E OÑATE SANCHEZ,
DIANA HERNÁNDEZ ROMERO
, MAR COLLADO GONZÁLEZ, JOSÉ LUIS CENIS,
JOSE MARIA MORALEDA JIMÉNEZ
,
Grupos de investigación:
[GI/IMIB/C061/2011] TRASPLANTE HEMATOPOYÉTICO / TERAPIA CELULAR
[GI/IMIB/C002/2011] CARDIOLOGIA CLINICA Y EXPERIMENTAL
Comunicación:
Antecedentes:
AIM: The purpose of this study was to investigate the effects of different composite films of graphene, silk fibroin and graphene combined with fibroin in the mesenchymal proliferation rate and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs).
Métodos:
Methods: hPDLSCs were cultured on graphene, silk fibroin or a combination of graphene and silk fibroin films up to 10 days. Then, the proliferation rate of the hPDLSCs was assessed using the MTT colorimetric assay. Also, gene expression for some osteogenic and cementogenic markers reported in the literature were assayed by qPCR: BMP2, RUNX2, SOX9, OSX, ALP, BGALP, DSPP, CEMP-1, CAP/PTPLA and GAPDH. Statistical analysis was carried out using SPSS statistical software version 19. Differences between multiple groups were analyzed by ANOVA with a Tukey post-test. p < 0.05 was deemed significant.
Resultados:
Results: At day 10 of culture, a significant increase in hPDLSCs proliferation rate on graphene films compared to plastic (p < 0.05) was observed, as well as on graphene plus fibroin compared to fibroin alone (p < 0.001). Concomitant up-regulation of RUNX2, SOX9 and OSX also suggested an intermediate chondrogenic differentiation similar to that reported during endochondral ossification. Noteworthy, the best results were obtained for graphene-coated plates, which resulted in the highest up-regulation of SP7/OSX and ALP. Moreover, the use of fibroin as substrate was of particular interest as it was associated with an over-expression of BMP2 and SP/OSX. Importantly, lone-reduced-graphene and the sandwich preparation including reduced graphene/fibroin, strongly promoted CEMP-1 over-expression.
Conclusiones:
Conclusion: Our findings support the potential of fibroin or graphene constructs as osteoinducers and vehicles to deliver PDLSCs or other dental stem cells.
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